BD FACSAria III
The BD FACSAria III is a revolutionary cell sorter known for superior multicolour performance across a range of research applications.
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A patented flow cell with gel-coupled cuvette and patented octagon and trigon detection system allow the system to achieve high sensitivity and resolution.
Has 4 lasers: 405, 488, 561 and 640 nm.
16 fluorescent channels available.
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Capable of ‘bulk sorting’ cells into 1.5mL Eppendorf’s, 5mL FACS tubes, or 15mL Falcon tubes, as well as single cell sorting into plates.
A choice of nozzle sizes are available: 70, 85, 100, 130 um.
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Due to its complexity - the BD FACSAria III can only be operated by a trained member of facility staff.
Use and applications
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Cell sorting speed is dependent on a number of factors: Initial sample concentration, the fequency of cells of interest to be sorted from the initial sample, the type of cell being sorted, the quality of cells provided in the sample, and the differential pressure applied to the cells. This means that cell sorting speeds are variable and determined on a case to case basis.
However, as an example: to get through 1mL of PBMCs at a concentration of 10 million cells/mL at the maximal differential pressure (highest speed) will take approximately 30 minutes.
Users are strongly advised to filter their cells prior to sorting and to suspend their samples in a solution containing EDTA to avoid cell clumping. Cell clumps can result in the cell sorters being clogged, resulting in extended sorting times. -
If you can see it - you can sort it!
Both fresh and fixed cells can be acquired but they must be biosafety level 1 status.
This cytometer can detect objects as small as 0.5uM beads, bacteria, and platelets using the FSCA (surface area) and SSCA (complexity) parameters.
Small objects with with sizes from 100nM - 50uM can be detected based on fluorescent tags.
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Please click here for a full list of costs and charges.
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Yes - Please book as far in advance as possible to ensure your time slot. However, It is often possible to find a timeslot on this machine for same day bookings.
The facility houses 2 sorters with identical optic pathways (Aria III and Aria Fusion). This means that there should always be a backup instrument available if one breaks down.
BD FACSAria Fusion
The fusion of safety, performance and sorting, the BD FACSAria Fusion is a state-of-the-art flow cytometer combining sorting capabilities with best-in-class biosafety expertise for a comprehensive advanced cell sorter and biosafety solution.
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A patented flow cell with gel-coupled cuvette and patented octagon and trigon detection system allow the system to achieve high sensitivity and resolution.
Has 5 lasers: UV 355, Violet 405, Blue 488, Yellow-Green 561 and Red 640 nm.
16 fluorescent channels available.
-
Capable of ‘bulk sorting’ cells into 1.5mL Eppendorf’s, 5mL FACS tubes, or 15mL Falcon tubes, as well as single cell sorting into plates.
A choice of nozzle sizes are available: 70, 85, 100, 130 um.
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Assisted only. The BD FACSAria Fusion can only be operated by a trained staff member.
Use and applications
-
Cell sorting speed is dependent on a number of factors: Initial sample concentration, the fequency of cells of interest to be sorted from the initial sample, the type of cell being sorted, the quality of cells provided in the sample, and the differential pressure applied to the cells. This means that cell sorting speeds are variable and determined on a case to case basis.
However, as an example: to get through 1mL of PBMCs at a concentration of 10 million cells/mL at the maximal differential pressure (highest speed) will take approximately 30 minutes.
Users are strongly advised to filter their cells prior to sorting and to suspend their samples in a solution containing EDTA to avoid cell clumping. Cell clumps can result in the cell sorters being clogged, resulting in extended sorting times.
-
If you can see it - you can sort it!
Both fresh and fixed cells can be acquired but they must be biosafety level 1 status.
This cytometer can detect objects as small as 0.5uM beads, bacteria, and platelets using the FSCA (surface area) and SSCA (complexity) parameters.
Small objects with with sizes from 100nM - 50uM can be detected based on fluorescent tags.
-
Please click here for a full list of costs and charges.
-
Yes - Please book as far in advance as possible to ensure your time slot. However, It is often possible to find a timeslot on this machine for same day bookings.
The facility houses 2 sorters with identical optic pathways (Aria III and Aria Fusion). This means that there should always be a backup instrument available if one breaks down.
Have a question?
Tel: +44 1865 285 479
Or send us a message using the contact form.